Coding

Part:BBa_K4060505

Designed by: Cheng-Ju Lu   Group: iGEM21_NYCU-Taipei   (2021-10-21)


BphP1 gene

The BphP1 gene cloned from Addgene plasmid pKA-207I10. bacterial phytochrome photoreceptor 1(BphP1) is NIR light transcription regulator. It undergoes NIR-light-triggered configuration transformation(Pfr state to Pr state) while under 740nm-780nm. Its natural protein parner is PpsP2. BphP1 and PpsR2 build up the BphP1-PpsR2 optogenetic system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 416
    Illegal XhoI site found at 2166
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 367
    Illegal NgoMIV site found at 402
    Illegal NgoMIV site found at 1650
    Illegal NgoMIV site found at 2152
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1207
    Illegal BsaI.rc site found at 2053


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Categories
Parameters
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